Apoptosis and Oxidative Stress Triggered by Carbon Black Nanoparticle in the LA-9 Fibroblast

BACKGROUND/AIMS: A new type of nanoparticle, called NP CB-EDA (Black Carbon modified with ethylenediamine), is commonly used in the oil industry. In literature, few studies are found biological models, making NP-EDA potential cytotoxicity organisms unclear. As its large surface area capable interacting system, that interaction could lead to factors harmful health. The objective this study was investigate cytotoxic effect on fibroblasts LA-9 at 24 and 48 hours, different concentrations nanoparticle (1, 50, 250, 500 1000 µg/ml). METHODS: characterized by TEM microscopy cell viability (MTT method), morphology (optical microscopy), membrane (lactate dehydrogenase release - LDH), oxidative stress pathways (species levels reactive oxygen, ROS nitrogen, NOS) apoptosis/necrosis (flow cytometry) were evaluated. RESULTS: results show µg/ml form clusters. can be absorbed cells decreasing viability. There damage LA 9, an increase production ROS, NOS pro-inflammatory interleukins TNF-α IL-6; it also observed % state apoptosis two periods analyzed, being response more significant presenting higher cytotoxicity. CONCLUSION: data suggest LA9 presents potential, which associated apoptosis.